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Friday, February 6, 2009

AIIMS May 2008 Physiology Question

Phosphatidyl choline in a lipid monolayer, at pH of 3.5 leads to ?

a) Decreased surface potential
b) Increased Surface potential
c) Decreased Dipole moment
d) Zero Dipole moment.

Answer : ? B

This article I found might throw some light.


"Solution pH Alters Mechanical and Electrical Properties of Phosphatidylcholine Membranes: Relation between Interfacial Electrostatics, Intramembrane Potential, and Bending Elasticity"
Biophys J. 2007 April 1; 92(7): 2451–2462.
Published online 2006 December 15. doi: 10.1529/biophysj.106.096362.

Yong Zhou and Robert M. Raphael

"the membrane dipole potential increases as solution pH decreases"
which should rule out the last two options.


Now read this ---------

"
At pH 2 and 3, SOPC vesicles have a positive ζ-potential, indicating extensive association of protons at the membrane surface. This agrees with other investigations demonstrating that phospholipids carry a positive ζ-potential at low pHs . At pH 4, the ζ-potential is nearly zero (∼3.5 mV) and the LUVs showed low mobility, in agreement with other investigations demonstrating that the isoelectric point of PC lipids is ∼pH 4 . At the near neutral pH of 6.5, the ζ-potential is clearly negative (∼−21 mV), in agreement with many investigations which have pointed out that PC is negatively charged between −10 mV and −30 mV at neutral pH"

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Therefore the answer might be (B). Increased Surface Potential



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